This study evaluated the PRP products from five centrifuged-based platforms: GS30- PurePRP® II and GS60-PurePRP® II, (EmCyte Corporation®), MAGELLAN, (Arteriocyte), REGENKIT®-THT (Stryker) and, ECLIPSE PRP (ECLIPSE Aesthetics) in a paired assay design with all five platforms tested with each donor. All systems were used according to manufacturer’s instruction for use with the most commonly used clinical method employed where alternate protocols were included. The REGEN and ECLIPSE platforms are vacuum tube systems containing a plasma separator gel. The Arteriocyte MAGELLAN system is a buffy coat system that harvests platelets from the surface of the RBC layer using an optical sensor. The two EmCyte PurePRP® systems use a dual spin process: the first step separates platelets from red cell using a soft spin that leaves the platelets in the plasma; the second spin concentrates the platelets and removes platelet poor plasma.