15 May 2015
To compare bone marrow concentrates produced with two commercial systems, the EmCyte GSBMA-544E and the Harvest/Terumo BMAC2.
Approximately 120mL of bone marrow aspirate was drawn from each of two donors prior to surgical procedure. For each donor, 50 mL of aspirate plus 5mL of Na Heparin (1000 U/mL) and 5mL of NaCitrate was processed with the EmCyte system according to manufacturer’s instructions for use. For each donor, 60mL of aspirate was added to the Harvest MarrowPREP Filter Bag along with 8mL of Anticoagulant Citrate Dextrose formula A and processed with the Harvest/Terumo SmartPREP 2 system according to manufacturer’s instructions for use. One mL samples of bone marrow concentrates aspirates were sent to the Testing Laboratory for the following analysis: Total Nucleated Cell Counts (TNC): determined with a Coulter AcT Diff2 hematology analyzer. Platelet Counts (PLT): determined with the Coulter analyzer CD34 Pos. Cells: CD34 Positive cells were measured using an Accuri C6 flow cytometer.